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low rox  (Biotium)


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    Structured Review

    Biotium low rox
    Low Rox, supplied by Biotium, used in various techniques. Bioz Stars score: 95/100, based on 195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/evagreen+qpcr+master+mix/Forget-Me-Not+EvaGreen+qPCR+Master+Mix/pmc13127909-150-27-30
    Average 95 stars, based on 195 article reviews
    low rox - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Sustained and reversible effects of a dietary phosphate intake on bone and mineral metabolism during aging.
    Article Snippet: The loss of bone volume during aging is common in both men and women and can have substantial negative health impacts.. Weakened bones can lead to fractures which in turn can result in hospitalization, decreased quality of life, and early death.. The post-diagnosis treatment of osteoporosis has received the bulk of attention with less research focused on prevention and modifiable risk factors such as nutrition.

    Synthesized:

    Article Title: Sustained and reversible effects of a dietary phosphate intake on bone and mineral metabolism during aging.
    Article Snippet: The loss of bone volume during aging is common in both men and women and can have substantial negative health impacts.. Weakened bones can lead to fractures which in turn can result in hospitalization, decreased quality of life, and early death.. The post-diagnosis treatment of osteoporosis has received the bulk of attention with less research focused on prevention and modifiable risk factors such as nutrition.

    Quantitative RT-PCR:

    Article Title: Sustained and reversible effects of a dietary phosphate intake on bone and mineral metabolism during aging.
    Article Snippet: The loss of bone volume during aging is common in both men and women and can have substantial negative health impacts.. Weakened bones can lead to fractures which in turn can result in hospitalization, decreased quality of life, and early death.. The post-diagnosis treatment of osteoporosis has received the bulk of attention with less research focused on prevention and modifiable risk factors such as nutrition.

    Article Title: BRD9 functions as a methylarginine reader to regulate AKT-EZH2 signaling.
    Article Snippet: .. For RT- qPCR, the iScript Reverse Transcription Supermix (Bio- Rad, 1708841) was used to synthesize the first- stand cDNA. qPCR assays were carried out using EvaGreen qPCR Master Mix (Biotium, 31041) with normalization to glyceraldehyde- 3- phosphate dehydrogenase (GAPDH). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Sustained and reversible effects of a dietary phosphate intake on bone and mineral metabolism during aging.
    Article Snippet: The loss of bone volume during aging is common in both men and women and can have substantial negative health impacts.. Weakened bones can lead to fractures which in turn can result in hospitalization, decreased quality of life, and early death.. The post-diagnosis treatment of osteoporosis has received the bulk of attention with less research focused on prevention and modifiable risk factors such as nutrition.

    Article Title: The Effect of Sex on Active and Latent Tuberculosis Occurrence Based on Mannose-Binding Lectin 2 Gene Expression and Mannose-binding Lectin Plasma Concentration in Indonesia.
    Article Snippet: The cDNA was synthesized using the iScript cDNA Synthesis Kit (Bio‐Rad Laboratories, USA). .. Relative gene expression was assessed using EvaGreen qPCR Master Mix (Biotium, USA) with GAPDH as the reference gene. .. International Journal of Mycobacteriology ¦ Volume 14 ¦ Issue 2 ¦ April-June 202598 Measurement of mannose‐binding lectin protein concentration MBL protein concentration was determined using the Human MBL ELISA Kit (Bioassay Technology, China).

    Article Title: Bacterial 16S Ribosomal Gene Fingerprints as a Tool to Diagnose and Mitigate Fish Larvae Gut Dysbiosis
    Article Snippet: Using the designed primer set, genomic extracts from the gut of larvae from good and bad quality batches were analysed in duplicate reactions by qPCR in a Bio‐Rad CFX96 instrument (Bio‐Rad Laboratories, Lisbon). .. The reaction volume was 10 μL and contained 200 nM of each primer, 80 ng of purified DNA (2 μL), or serial dilutions of the miniprep as the template (corresponding to 10 2 –10 7 template copies per reaction) for the standard curve, 5 μL of 2× Forget‐Me‐Not EvaGreen qPCR Master Mix (Biotium) and 2.4 μL of sterile nuclease‐free water. ..

    Article Title: Impact of Promoter-Independent CpG-Rich A2UCOE Subregions on Transgene Expression
    Article Snippet: .. A single-copy A2UCOE-eGFP LV cell clone, isolated via limiting dilution from bulk-transduced P19 and F9 cells, was used as a reference standard. qPCR was carried out using EvaGreen® qPCR Master Mix (Biotium, CA, USA), in accordance with the manufacturer’s instructions. ..

    Article Title: ACAN and VCAN Inhibition Through Downregulation of SOX9 and Induction of Neurogenesis in Rat Using Ischemic Stroke Model Due to Simvastatin Administration
    Article Snippet: .. KG Widayat et al.: Simvastatin Induces Neurogenesis Via SOX9 Downregulation in Stroke Rats NotTM EvaGreen® qPCR Master Mix (Low ROX) (Catalog No. 31045-1mL, 31045-5mL, 31045-20mL; Biotium, Fremont, CA, USA). ..

    Article Title: Senomorphic Activity of a Novel Standardized Propolis Extract in Human Dermal Fibroblasts: Molecular Insights Into Clinically Proven Anti‐Wrinkle Efficacy
    Article Snippet: Total RNA was extracted from HDFs using the RNeasy Mini kit (Qiagen). cDNA was synthesized using the High‐Capacity RNA‐to‐cDNA Kit (ThermoFisher, Cat. #4387406). .. Real‐time quantitative PCR was performed on a Bio‐Rad C1000 Touch System with CFX384 optical module using Forget‐Me‐Not EvaGreen qPCR Master Mix (Biotium, Lot #23F1213) and commercially validated primer sets (RealTimePrimers, Elkins Park, PA). ..

    Article Title: BRD9 functions as a methylarginine reader to regulate AKT-EZH2 signaling.
    Article Snippet: .. For RT- qPCR, the iScript Reverse Transcription Supermix (Bio- Rad, 1708841) was used to synthesize the first- stand cDNA. qPCR assays were carried out using EvaGreen qPCR Master Mix (Biotium, 31041) with normalization to glyceraldehyde- 3- phosphate dehydrogenase (GAPDH). ..

    Article Title: BRD9 functions as a methylarginine reader to regulate AKT-EZH2 signaling
    Article Snippet: .. For RT-qPCR, the iScript Reverse Transcription Supermix (Bio-Rad, 1708841) was used to synthesize the first-stand cDNA. qPCR assays were carried out using EvaGreen qPCR Master Mix (Biotium, 31041) with normalization to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). ..

    Gene Expression:

    Article Title: The Effect of Sex on Active and Latent Tuberculosis Occurrence Based on Mannose-Binding Lectin 2 Gene Expression and Mannose-binding Lectin Plasma Concentration in Indonesia.
    Article Snippet: The cDNA was synthesized using the iScript cDNA Synthesis Kit (Bio‐Rad Laboratories, USA). .. Relative gene expression was assessed using EvaGreen qPCR Master Mix (Biotium, USA) with GAPDH as the reference gene. .. International Journal of Mycobacteriology ¦ Volume 14 ¦ Issue 2 ¦ April-June 202598 Measurement of mannose‐binding lectin protein concentration MBL protein concentration was determined using the Human MBL ELISA Kit (Bioassay Technology, China).

    Purification:

    Article Title: Bacterial 16S Ribosomal Gene Fingerprints as a Tool to Diagnose and Mitigate Fish Larvae Gut Dysbiosis
    Article Snippet: Using the designed primer set, genomic extracts from the gut of larvae from good and bad quality batches were analysed in duplicate reactions by qPCR in a Bio‐Rad CFX96 instrument (Bio‐Rad Laboratories, Lisbon). .. The reaction volume was 10 μL and contained 200 nM of each primer, 80 ng of purified DNA (2 μL), or serial dilutions of the miniprep as the template (corresponding to 10 2 –10 7 template copies per reaction) for the standard curve, 5 μL of 2× Forget‐Me‐Not EvaGreen qPCR Master Mix (Biotium) and 2.4 μL of sterile nuclease‐free water. ..

    Sterility:

    Article Title: Bacterial 16S Ribosomal Gene Fingerprints as a Tool to Diagnose and Mitigate Fish Larvae Gut Dysbiosis
    Article Snippet: Using the designed primer set, genomic extracts from the gut of larvae from good and bad quality batches were analysed in duplicate reactions by qPCR in a Bio‐Rad CFX96 instrument (Bio‐Rad Laboratories, Lisbon). .. The reaction volume was 10 μL and contained 200 nM of each primer, 80 ng of purified DNA (2 μL), or serial dilutions of the miniprep as the template (corresponding to 10 2 –10 7 template copies per reaction) for the standard curve, 5 μL of 2× Forget‐Me‐Not EvaGreen qPCR Master Mix (Biotium) and 2.4 μL of sterile nuclease‐free water. ..

    Isolation:

    Article Title: Impact of Promoter-Independent CpG-Rich A2UCOE Subregions on Transgene Expression
    Article Snippet: .. A single-copy A2UCOE-eGFP LV cell clone, isolated via limiting dilution from bulk-transduced P19 and F9 cells, was used as a reference standard. qPCR was carried out using EvaGreen® qPCR Master Mix (Biotium, CA, USA), in accordance with the manufacturer’s instructions. ..

    Reverse Transcription:

    Article Title: BRD9 functions as a methylarginine reader to regulate AKT-EZH2 signaling.
    Article Snippet: .. For RT- qPCR, the iScript Reverse Transcription Supermix (Bio- Rad, 1708841) was used to synthesize the first- stand cDNA. qPCR assays were carried out using EvaGreen qPCR Master Mix (Biotium, 31041) with normalization to glyceraldehyde- 3- phosphate dehydrogenase (GAPDH). ..

    Article Title: BRD9 functions as a methylarginine reader to regulate AKT-EZH2 signaling
    Article Snippet: .. For RT-qPCR, the iScript Reverse Transcription Supermix (Bio-Rad, 1708841) was used to synthesize the first-stand cDNA. qPCR assays were carried out using EvaGreen qPCR Master Mix (Biotium, 31041) with normalization to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). ..



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    (A) CryoEM map of the Cbf1:CCAN complex bound to a fragment of C0N3 DNA containing the CDEI element (PDB: 8OVW). The interaction between Cbf1 and Okp1 is detailed in the inset. (B) Flag-tag immunoprecipitations of Cbf1-3Flag (SBY18421) and Cbf1-EW-3Flag (L283E, L287W; SBY22227) were immunoblotted against Okp1 to analyze co-purifying levels. (C) Immunoblots of DNA-bound proteins from de novo kinetochore assembly assays performed using extracts from asynchronously grown CBF1-3FLAG (SBY18421), cbf1Δ (SBY4958), and cbf1-EW-3FLAG (SBY22227) strains with the indicated DNA templates. (D) Tetrad dissection of a cross between cbf1-EW (SBY22227) and dsn1-3A (SBY14171) strains. The four spores from individual asci are aligned in horizontal rows. Orange circles represent spores with double mutant genotype. (E) Schematic of the TIRFM stability assay. Lysate is incubated on the TIRFM slide for 5 or 90 minutes before being washed off. Slides were then either imaged immediately or after 20 minutes. (F) Percentages of colocalization between CEN3 DNAs and Cbf1-GFP (SBY22129), Cbf1-EW-GFP (SBY22923) and Cbf1-GFP in ctf19Δ cells (SBY24889) as analyzed by TIRFM after 5 min or 90 min of incubation and imaged immediately post wash. Error bars represent the standard deviation over three biological repeats. At least 3000 DNA molecules were imaged for each biological replicate. (G) Same as in (E) but imaged 20 minutes post wash. (H) <t>RT-qPCR</t> analysis of cenRNA expression of CEN4, CEN5 , and CEN8 in wild type (SBY22452), cbf1Δ (SBY22454), and cbf1- EW (SBY22456) cells arrested in G1 with α-Factor. Expression levels were quantified relative to that of wild type (mean ± SD, n=3). Statistical significances were analyzed by unpaired t-tests (*, p<0.05; **, p<0.01).
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    (A) CryoEM map of the Cbf1:CCAN complex bound to a fragment of C0N3 DNA containing the CDEI element (PDB: 8OVW). The interaction between Cbf1 and Okp1 is detailed in the inset. (B) Flag-tag immunoprecipitations of Cbf1-3Flag (SBY18421) and Cbf1-EW-3Flag (L283E, L287W; SBY22227) were immunoblotted against Okp1 to analyze co-purifying levels. (C) Immunoblots of DNA-bound proteins from de novo kinetochore assembly assays performed using extracts from asynchronously grown CBF1-3FLAG (SBY18421), cbf1Δ (SBY4958), and cbf1-EW-3FLAG (SBY22227) strains with the indicated DNA templates. (D) Tetrad dissection of a cross between cbf1-EW (SBY22227) and dsn1-3A (SBY14171) strains. The four spores from individual asci are aligned in horizontal rows. Orange circles represent spores with double mutant genotype. (E) Schematic of the TIRFM stability assay. Lysate is incubated on the TIRFM slide for 5 or 90 minutes before being washed off. Slides were then either imaged immediately or after 20 minutes. (F) Percentages of colocalization between CEN3 DNAs and Cbf1-GFP (SBY22129), Cbf1-EW-GFP (SBY22923) and Cbf1-GFP in ctf19Δ cells (SBY24889) as analyzed by TIRFM after 5 min or 90 min of incubation and imaged immediately post wash. Error bars represent the standard deviation over three biological repeats. At least 3000 DNA molecules were imaged for each biological replicate. (G) Same as in (E) but imaged 20 minutes post wash. (H) <t>RT-qPCR</t> analysis of cenRNA expression of CEN4, CEN5 , and CEN8 in wild type (SBY22452), cbf1Δ (SBY22454), and cbf1- EW (SBY22456) cells arrested in G1 with α-Factor. Expression levels were quantified relative to that of wild type (mean ± SD, n=3). Statistical significances were analyzed by unpaired t-tests (*, p<0.05; **, p<0.01).
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    Image Search Results


    Male and female hACE2/Poldip2 mice were infected with SARS-CoV-2 by nasal inoculation. Lungs were collected 7 days later for RNA preparation. Viral loads were measured by RT-qPCR using primer pairs and TaqMan probes specific for the viral gene N (A) or subgenomic E (sgE) RNA (B). Bars represent means ± SEM of data from n = 5-7 animals. Groups were compared using two-tailed Mann-Whitney tests: **P < 0.01.

    Journal: PLOS One

    Article Title: Poldip2 deficiency attenuates lung disease severity in a mouse model of COVID-19

    doi: 10.1371/journal.pone.0348065

    Figure Lengend Snippet: Male and female hACE2/Poldip2 mice were infected with SARS-CoV-2 by nasal inoculation. Lungs were collected 7 days later for RNA preparation. Viral loads were measured by RT-qPCR using primer pairs and TaqMan probes specific for the viral gene N (A) or subgenomic E (sgE) RNA (B). Bars represent means ± SEM of data from n = 5-7 animals. Groups were compared using two-tailed Mann-Whitney tests: **P < 0.01.

    Article Snippet: Reverse transcription was performed using Protoscript II reverse transcriptase (M0368, New England Biolabs) with random primers. cDNA was amplified with 2X Forget-Me-Not EvaGreen qPCR Master Mix with Low ROX (31045, Biotium) and primers against mouse TNFα, IL-1β, IL-6, MCP1, CXCL1, IFN-γ, Poldip2 and human ACE2 ( ).

    Techniques: Infection, Quantitative RT-PCR, Two Tailed Test, MANN-WHITNEY

    Male and female hACE2/Poldip2 mice were euthanized 7 days after intranasal administration of PBS or SARS-CoV-2. Bronchoalveloar lavage (BAL) fluid was collected before harvesting lungs for RNA extraction as in Figure 3. mRNAs were measured by RT-qPCR in lung tissue (A) and protein ELISAs were carried out in BAL (B). Bars represent means ± SEM of data from n = 4-17 animals. Basal protein levels in +/ + PBS were 40 ± 20 pg/ml (MCP1) and 111 ± 31 pg/ml (CXCL1). Data were analyzed using 2-way ANOVA: ns, not significant; ** P < 0.01; *** P < 0.001.

    Journal: PLOS One

    Article Title: Poldip2 deficiency attenuates lung disease severity in a mouse model of COVID-19

    doi: 10.1371/journal.pone.0348065

    Figure Lengend Snippet: Male and female hACE2/Poldip2 mice were euthanized 7 days after intranasal administration of PBS or SARS-CoV-2. Bronchoalveloar lavage (BAL) fluid was collected before harvesting lungs for RNA extraction as in Figure 3. mRNAs were measured by RT-qPCR in lung tissue (A) and protein ELISAs were carried out in BAL (B). Bars represent means ± SEM of data from n = 4-17 animals. Basal protein levels in +/ + PBS were 40 ± 20 pg/ml (MCP1) and 111 ± 31 pg/ml (CXCL1). Data were analyzed using 2-way ANOVA: ns, not significant; ** P < 0.01; *** P < 0.001.

    Article Snippet: Reverse transcription was performed using Protoscript II reverse transcriptase (M0368, New England Biolabs) with random primers. cDNA was amplified with 2X Forget-Me-Not EvaGreen qPCR Master Mix with Low ROX (31045, Biotium) and primers against mouse TNFα, IL-1β, IL-6, MCP1, CXCL1, IFN-γ, Poldip2 and human ACE2 ( ).

    Techniques: RNA Extraction, Quantitative RT-PCR

    (A) CryoEM map of the Cbf1:CCAN complex bound to a fragment of C0N3 DNA containing the CDEI element (PDB: 8OVW). The interaction between Cbf1 and Okp1 is detailed in the inset. (B) Flag-tag immunoprecipitations of Cbf1-3Flag (SBY18421) and Cbf1-EW-3Flag (L283E, L287W; SBY22227) were immunoblotted against Okp1 to analyze co-purifying levels. (C) Immunoblots of DNA-bound proteins from de novo kinetochore assembly assays performed using extracts from asynchronously grown CBF1-3FLAG (SBY18421), cbf1Δ (SBY4958), and cbf1-EW-3FLAG (SBY22227) strains with the indicated DNA templates. (D) Tetrad dissection of a cross between cbf1-EW (SBY22227) and dsn1-3A (SBY14171) strains. The four spores from individual asci are aligned in horizontal rows. Orange circles represent spores with double mutant genotype. (E) Schematic of the TIRFM stability assay. Lysate is incubated on the TIRFM slide for 5 or 90 minutes before being washed off. Slides were then either imaged immediately or after 20 minutes. (F) Percentages of colocalization between CEN3 DNAs and Cbf1-GFP (SBY22129), Cbf1-EW-GFP (SBY22923) and Cbf1-GFP in ctf19Δ cells (SBY24889) as analyzed by TIRFM after 5 min or 90 min of incubation and imaged immediately post wash. Error bars represent the standard deviation over three biological repeats. At least 3000 DNA molecules were imaged for each biological replicate. (G) Same as in (E) but imaged 20 minutes post wash. (H) RT-qPCR analysis of cenRNA expression of CEN4, CEN5 , and CEN8 in wild type (SBY22452), cbf1Δ (SBY22454), and cbf1- EW (SBY22456) cells arrested in G1 with α-Factor. Expression levels were quantified relative to that of wild type (mean ± SD, n=3). Statistical significances were analyzed by unpaired t-tests (*, p<0.05; **, p<0.01).

    Journal: bioRxiv

    Article Title: An interdependent Cbf1-CCAN interaction stabilizes the budding yeast kinetochore

    doi: 10.64898/2026.03.25.714319

    Figure Lengend Snippet: (A) CryoEM map of the Cbf1:CCAN complex bound to a fragment of C0N3 DNA containing the CDEI element (PDB: 8OVW). The interaction between Cbf1 and Okp1 is detailed in the inset. (B) Flag-tag immunoprecipitations of Cbf1-3Flag (SBY18421) and Cbf1-EW-3Flag (L283E, L287W; SBY22227) were immunoblotted against Okp1 to analyze co-purifying levels. (C) Immunoblots of DNA-bound proteins from de novo kinetochore assembly assays performed using extracts from asynchronously grown CBF1-3FLAG (SBY18421), cbf1Δ (SBY4958), and cbf1-EW-3FLAG (SBY22227) strains with the indicated DNA templates. (D) Tetrad dissection of a cross between cbf1-EW (SBY22227) and dsn1-3A (SBY14171) strains. The four spores from individual asci are aligned in horizontal rows. Orange circles represent spores with double mutant genotype. (E) Schematic of the TIRFM stability assay. Lysate is incubated on the TIRFM slide for 5 or 90 minutes before being washed off. Slides were then either imaged immediately or after 20 minutes. (F) Percentages of colocalization between CEN3 DNAs and Cbf1-GFP (SBY22129), Cbf1-EW-GFP (SBY22923) and Cbf1-GFP in ctf19Δ cells (SBY24889) as analyzed by TIRFM after 5 min or 90 min of incubation and imaged immediately post wash. Error bars represent the standard deviation over three biological repeats. At least 3000 DNA molecules were imaged for each biological replicate. (G) Same as in (E) but imaged 20 minutes post wash. (H) RT-qPCR analysis of cenRNA expression of CEN4, CEN5 , and CEN8 in wild type (SBY22452), cbf1Δ (SBY22454), and cbf1- EW (SBY22456) cells arrested in G1 with α-Factor. Expression levels were quantified relative to that of wild type (mean ± SD, n=3). Statistical significances were analyzed by unpaired t-tests (*, p<0.05; **, p<0.01).

    Article Snippet: 1 μg of DNase-treated RNA was reverse transcribed using RevertAid Reverse Transcriptase (Thermo Fisher Scientific, #EP0442) in a 20 μL reaction using random hexamer and oligo(dT)18 primers and analyzed by qPCR using the Forget-Me-Not EvaGreen qPCR Master Mix (Biotium, #31045) with primers listed in Supplemental Table S3. qPCR was performed using a Quantstudio TM 5 Real-Time PCR System (Applied Biosystem).

    Techniques: FLAG-tag, Western Blot, Dissection, Mutagenesis, Stability Assay, Incubation, Standard Deviation, Quantitative RT-PCR, Expressing